Image obtained by researchers from the Oswaldo Cruz Institute (IOC/Fiocruz) shows the moment when a cell undergoes a degeneration process after infection by the monkeypox virus. [high resolution photos in the link at the end of the article]
Change in the cell caused by the viral infection. Photo: Milene Dias Miranda/IOC/Fiocruzi
To capture the cytopathic effect in a monolayer, as it is scientifically called, a clinical sample of an infected patient was inoculated into Vero lineage cells, a model frequently used for in vitro assays and viral isolation. This procedure was performed in a level 3 biosafety laboratory (NBA3) of the IOC.
In another click, obtained with a transmission electron microscope, it is possible to visualize in an enlarged way the ultrastructure of the cell after replication of the pathogen, more precisely, infecting the cytoplasm, the region where the nucleus is found, responsible for storing the cell's genetic material.
Viral particles replicate inside the cell. Photo: Débora F. Barreto-Vieira/IOC/Fiocruzi
With the image magnification of 40 times, it is possible to identify more closely the viral particles in the process of replication in the cell's cytoplasm.
From the images, it could be verified that despite the reduced size of the virus in relation to the cell – which is 300 times larger – it is capable of infecting the structure and replicating with ease.
From the records, the monkeypox virus is estimated to measure 300 nanometers on average.The research “Isolation and ultrastructural studies of monkeypox virus in Vero cells from clinical samples” is coordinated by the head of the Laboratory of Viral Morphology and Morphogenesis, Debora Ferreira Barreto Vieira, with the collaboration of her team (Milene Dias Miranda, Gabriela Cardoso Caldas and Vivian Ferreira), in partnership with the team from the Enterovirus Laboratory, headed by Edson Elias, who acts as a reference in laboratory diagnosis of monkeypox for the Ministry of Health, and who was responsible for viral detection in the sample used in the study.
Reproduction of images is authorized with full citation of the source:
:: Débora F. Barreto-Vieira/IOC/Fiocruz (for photographs that show viral particles)
:: Milene Dias Miranda/IOC/Fiocruz (for the inverted microscope image).
Image obtained by researchers from the Oswaldo Cruz Institute (IOC/Fiocruz) shows the moment when a cell undergoes a degeneration process after infection by the monkeypox virus. [high resolution photos in the link at the end of the article]
Change in the cell caused by the viral infection. Photo: Milene Dias Miranda/IOC/Fiocruzi
To capture the cytopathic effect in a monolayer, as it is scientifically called, a clinical sample of an infected patient was inoculated into Vero lineage cells, a model frequently used for in vitro assays and viral isolation. This procedure was performed in a level 3 biosafety laboratory (NBA3) of the IOC.
In another click, obtained with a transmission electron microscope, it is possible to visualize in an enlarged way the ultrastructure of the cell after replication of the pathogen, more precisely, infecting the cytoplasm, the region where the nucleus is found, responsible for storing the cell's genetic material.
Viral particles replicate inside the cell. Photo: Débora F. Barreto-Vieira/IOC/Fiocruzi
With the image magnification of 40 times, it is possible to identify more closely the viral particles in the process of replication in the cell's cytoplasm.
From the images, it could be verified that despite the reduced size of the virus in relation to the cell – which is 300 times larger – it is capable of infecting the structure and replicating with ease.
From the records, the monkeypox virus is estimated to measure 300 nanometers on average.The research “Isolation and ultrastructural studies of monkeypox virus in Vero cells from clinical samples” is coordinated by the head of the Laboratory of Viral Morphology and Morphogenesis, Debora Ferreira Barreto Vieira, with the collaboration of her team (Milene Dias Miranda, Gabriela Cardoso Caldas and Vivian Ferreira), in partnership with the team from the Enterovirus Laboratory, headed by Edson Elias, who acts as a reference in laboratory diagnosis of monkeypox for the Ministry of Health, and who was responsible for viral detection in the sample used in the study.
Reproduction of images is authorized with full citation of the source:
:: Débora F. Barreto-Vieira/IOC/Fiocruz (for photographs that show viral particles)
:: Milene Dias Miranda/IOC/Fiocruz (for the inverted microscope image).
The non-profit reproduction of the text is allowed as long as the source is cited (Comunicação / Instituto Oswaldo Cruz)
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